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Biuret reagent is essential in biochemical analysis, serving a key role in both qualitative and quantitative protein detection. This comprehensive guide explores its composition, preparation, underlying principles, applications, and significance of the Biuret test, a vital technique used in laboratories globally. What is the Biuret Test? The biuret test is a chemical test that can be used to check for the presence of peptide bonds in a given analyte. Therefore, the biuret test can also be used to gauge the amount of protein present in the analyte. The biuret test is a colorimetric assay that detects the presence of at least two peptide bonds in a molecule, such as proteins. It involves a copper (II) ion forming a mauve complex with peptides in an alkaline solution, and can be modified by BCA or Lowry reagents for higher sensitivity. Biuret is created by heating urea at 180 degrees Celsius. The Biuret test is a chemical test that employs Biuret reagents, which include a 1% solution of Copper II sulphate (CuSO4). Cu2+ in the Biuret reagent produces a compound with protein peptide links. Therefore, this technique is useful for identifying peptide bonds in any substance. Peptide bonds are formed when two acids are linked by carbonyl and amino groups. In addition, the fundamental unit of protein is composed of amino acids ...
