Benedict’s test is a chemical test used to detect the presence of reducing sugars, such as glucose, in a solution. It involves heating the sample with Benedict’s reagent (a blue solution containing copper sulfate and sodium citrate) in a boiling water bath. When exposed to reducing sugars, the reactions undergone by Benedict’s reagent result in the formation of a brick-red precipitate, which indicates a positive Benedict’s test. Benedict's Test is a qualitative test for simple carbohydrates that have reducing properties. Learn how to prepare, perform and interpret the test, and see examples of positive and negative results. A positive result of Benedict's test is indicated by a color change from clear blue to brick-red with a precipitate. Generally, Benedict's test detects the presence of aldehyde groups, alpha-hydroxy-ketones, and hemiacetals, including those that occur in certain ketoses.

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